M. Mateu et al. Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma, Cancers

M. Mateu-Sanz, J Tornín, B. Brulin, A. Khlyustova, M.P. Ginebra , P. Layrolle, C. Canal. Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma, Cancers 2020, Vol. 12, Page 227, vol. 12, no. 1, p. 227.

doi: 10.3390/cancers12010227

Abstract

Osteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and dicult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer eects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the eects of plasma-treated culture media on cell cultures. However, eects of plasma-activated saline solutions with clinical application have not yet been explored in OS. The aim of this study is to obtain mechanistic insights on the action of plasma-activated Ringer’s saline (PAR) for OS therapy in cell and organotypic cultures. To that aim, cold atmospheric plasma jets were used to obtain PAR, which produced cytotoxic eects in human OS cells (SaOS-2, MG-63, and U2-OS), related to the increasing concentration of reactive oxygen and nitrogen species generated. Proof of selectivity was found in the sustained viability of hBM-MSCs with the same treatments. Organotypic cultures of murine OS confirmed the time-dependent cytotoxicity observed in 2D. Histological analysis showed a decrease in proliferating cells (lower Ki-67 expression). It is shown that the selectivity of PAR is highly dependent on the concentrations of reactive species, being the dierential intracellular reactive oxygen species increase and DNA damage between OS cells and hBM-MSCs key mediators for cell apoptosis.